Brain and Thymus Lipid Inhibition of Antibrain-associated Θ-cytotoxicity

Reif and Allen (1-3) demonstrated that 0-antigen was present on the surface of mouse thymocytes and a number of murine leukemia cells, in brain tissue and, to a lesser extent, in lymph nodes and spleen. They later showed that 0-antigen was controlled by a single locus with two alleles: 8-AKR found in AKR mice, and 0-C3H found in most inbred strains of mice, including CBA. Golub (4) has employed the fact that 0-antigen is present in brain to produce an antibrain-associated 0-antiserum (BA0) 1 by injecting mouse brain into rabbits. The activity of BA0 antiserum parallels that of AKR anti-0 C3H and C3H anti-0 AKR. The relationship between O-C3H, 8-AKR, and BA0 has recently been shown by Clagett et al. (5) and Peter et al. (6) to consist of a complex system of shared and unshared antigens between mouse 8-C3H, 0-AKR, and rat Lewis (Le) thymocytes.